Multiphoton Microscopy

Edmund Optics' partner, TMC, provides optical tables that excel in vibration isolation and offer several practical features ideal for biological research environments. These features include retractable casters for easy repositioning within the lab, a proprietary nylon cup structure that prevents water and other liquids from damaging the table, and ample storage space underneath for essential equipment.

Some advanced twin-microscope systems integrate one-photon and multiphoton imaging techniques using continuous wave (CW) visible lasers alongside ultrafast near-infrared lasers. In the Lattice Light Sheet Microscope (LLSM), CW lasers provide a stable source for one-photon excitation, enhancing image contrast and reducing photodamage to sensitive biological specimens. This combination allows for faster imaging speeds and longer observation times while minimizing phototoxicity associated with broader illumination methods.

Low Group Delay Dispersion (GDD) Ultrafast Mirrors steer and reflect ultrafast beams without adding any temporal dispersion to the laser pulse. Compared to ultrafast silver-coated mirrors, Low GDD Mirrors provide higher reflectance and higher damage thresholds, making them better suited for high-power femtosecond lasers.

Dichroic filters, in particular, are commonly used to separate excitation wavelengths from emission wavelengths.

Steering mirrors and optical scanners enable you to add extra dimensions to your measurements or process samples more efficiently. Samples are often scanned laterally or even axially, creating cross-sections that, when superimposed, provide high-resolution details.

Zaber™ Linear Motor XY Microscope Motorized Stages and Nanopositioning System provide an additional range of motion solutions for sample movement and dimensional scanning.

Microscope objectives are vital for delivering focused light to excite fluorophores in biological tissues. Key factors include a High Numerical Aperture (NA) for concentrating laser pulses and Long Working Distance or Water Immersion features, which maintain optical clarity and reduce spherical aberrations. Always check the objective's transmission for both excitation and emission wavelengths.

A photomultiplier tube (PMT), imaging lens and camera, or other detector setup collects the fluorescence emitted from the focal plane, resulting in high-resolution images. 3D images can be generated by scanning through different planes of focus and stitching the images together.

Selecting the appropriate microscopy camera involves considering several essential parameters, including resolution, sensor type, pixel size, frame rate, dynamic range, and spectral sensitivity. Different camera features can significantly impact image quality and usability depending on your specific research goals. We offer a wide selection of camera solutions—don’t hesitate to contact our engineers for assistance in finding the best solution for your application.